| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-130SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-448SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86946-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Ubiquitination of PML in response to As2O3. PML-/- MEFs were transiently transfected with the empty vector (EV) or PMLIII (left panel) or contructs encoding PMLIII, PMLIII-SIM or PMLVI (right panel). Two days later, they were treated with As2O3 for 1 h. The cell extracts were immunoprecipitated with rabbit anti-PML antibody and the immunoprecipitate was analyzed by Western blot using anti-ubiquitin and anti-PML antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044949) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0014.jpg "Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Ubiquitination of PML in response to As2O3. PML-/- MEFs were transiently transfected with the empty vector (EV) or PMLIII (left panel) or contructs encoding PMLIII, PMLIII-SIM or PMLVI (right panel). Two days later, they were treated with As2O3 for 1 h. The cell extracts were immunoprecipitated with rabbit anti-PML antibody and the immunoprecipitate was analyzed by Western blot using anti-ubiquitin and anti-PML antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044949) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody (Ubi-1) [NB300-130] - Study of mutant BRAF protein found within the inclusions. BRAFV600E/ubiquitin double-IF labeling. A nucleus with a large inclusion is shown with positive immunoreactivity for both mutant BRAF (red staining) and ubiquitin (green staining); the merged color yellow (arrow) points out the co-localisation of BRAFV600E and ubiquitin in the same inclusion. Image collected and cropped by CiteAb from the following publication (////doi.org/10.1371/journal.pone.0226199) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunocytochemistry-Immunofluorescence-NB300-130-img0013.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Analysis of HEK293 cell lysates using mouse mAb Ubiquitin, dilution 1:1000 (Green). [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with 10uM of proteasome inhibitor lactacystin (Lc) for 16hrs. Lysed cells were electrophoresed on 4-20% SDS-PAGE and transferred to PVDF membranes. The smear detected above the 200kDa standard represent accumulation of ubiquitinated proteins in proteasome inhibitor-Lc treated cells. The prominent band at 8kDa corresponds to monoubiquitin. Rabbit pAb to HSP60, dilution 1:5000 (Red) was used as a loading control.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0012.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - FFPE section of cerebral cortex of an Alzheimer patient processed with Ubiquitin antibody using HRP/DAB (Brown) and also stained with haemotoxylin (Blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0011.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0008.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Molecular Analysis of XopD Ub/Ubl Specificity. S. lycopersicum protein extract blotted for total Ub (Ubiquitin; Novus Biologicals) following a 1 hr room temperature treatment with the XopD variants used in (K) at 1 uM final concentration. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27425412/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0015.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Western-Blot-NB300-130-img0009.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Ubi-1-Immunohistochemistry-Paraffin-NB300-130-img0010.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gamma H2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-130_mouse-monoclonal-ubiquitin-antibody-ubi-1-imgenex-img-5021-310202416205188.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gamma H2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-130_mouse-monoclonal-ubiquitin-antibody-ubi-1-imgenex-img-5021-310202416205134.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - HUWE1 Assembles K6 Chains In Vitro & In Vivo(A) Table summarizing proteins identified with the corresponding number peptide-spectrum matches (PSMs) in three replicates of DUB-treated K6 affimer pull-downs.(B) In vitro assembly reaction of the HECT E3 HUWE1 with Ub WT, Ub K6R, Ub K11R, & Ub K48R on Coomassie. Arrows indicate K6 diUb.(C) Linkage composition of HUWE1-generated diUb after 1 hr as determined by AQUA MS.(D) AQUA-derived total cellular chain composition of HUWE1−/− HeLa & parental cells after TUBE-based enrichment. Error bars indicate mean ± SD from n = 3. ∗p < 0.05, according to a two-tailed Student’s t test. N.S., not significant.(E) TUBE-PD from a doxycycline-inducible HUWE1 shRNA Ls174T cell line blotted with the K6-specific affimer, with input controls for actin, total Ub, & HUWE1.(F) K6 affimer pull-down in doxycycline-inducible HUWE1 shRNA Ls174T cells blotted against Mfn2. Cells were left untreated or treated with 10 μg/mL MG132 for 4 hr and/or 1 μg/mL doxycycline for 72 hr. Pull-downs were incubated with 250 nM USP21 as indicated, with a K48 blot to show completeness of the deubiquitination reaction. Input controls are shown for Mfn2, actin, & HUWE1.See also Figure S7. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-130_mouse-monoclonal-ubiquitin-antibody-ubi-1-imgenex-img-5021-3102024162181.jpg)
![Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gamma H2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-130_mouse-monoclonal-ubiquitin-antibody-ubi-1-imgenex-img-5021-310202416205121.jpg)
![Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Western-Blot-NB300-129-img0009.jpg "Western Blot: Ubiquitin Antibody [NB300-129] - Analysis of HEK293 cell lysates using rabbit Ubiquitin antibody, dilution 1:5000 (Green), and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10uM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Analysis of HeLa cells stained with rabbit pAb to ubiquitin, NB300-129, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10uM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0008.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody [NB300-129] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunohistochemistry-Paraffin-NB300-129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NB300-129] - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using NB300-129.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-129-img0001.jpg)
![Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Western-Blot-NB100-448-img0005.jpg "Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.")
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-448-img0001.jpg)
![Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-NB100-448-img0006.jpg)
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0013.jpg "Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: USP14 Antibody (6D6) [H00009097-M04] - Analysis of monoclonal antibody to USP14 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Immunocytochemistry-Immunofluorescence-H00009097-M04-img0008.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0010.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0011.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0012.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody (M04), clone 6D6. Lane 1: USP14 transfected lysatE (56.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0014.jpg)
![ELISA: USP14 Antibody (6D6) [H00009097-M04] - Detection limit for recombinant GST tagged USP14 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-ELISA-H00009097-M04-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: USP14 Antibody [NBP1-86946] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/USP14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86946-img0009.jpg "Immunocytochemistry/Immunofluorescence: USP14 Antibody [NBP1-86946] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-86946_rabbit-polyclonal-usp14-antibody-immunohistochemistry-paraffin-1810202384223..jpg)
![Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human lymph node shows strong cytoplasmic and membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86946_rabbit-polyclonal-usp14-antibody-immunohistochemistry-paraffin-1810202384347..jpg)
![Immunohistochemistry-Paraffin: USP14 Antibody [NBP1-86946] - Staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-86946_rabbit-polyclonal-usp14-antibody-immunohistochemistry-paraffin-1810202384610..jpg)
![Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Western-Blot-NB600-776-img0002.jpg "Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.")
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Staining of human tonsil.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0003.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Human hypophysis.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0001.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419592087.jpg "Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202420312999.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241932686.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in HeLa Cells.Immunofluorescent analysis of PFA-fixed HeLa cells labeling Nanog with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419553033.jpg)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in Mouse and Rat Testis.Antibodies: Nanog, 2 u/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241923639.gif)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in HeLa CellsLoading: 15 u of lysate. Antibodies: Nanog, 2 u/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419463442.gif)
![Western Blot: USP33 Antibody [NB110-40693] - Detection of Human USP33 on HeLa whole cell lysate using NB110-40693. USP33 was also immunoprecipitated by rabbit anti-USP33 antibody NB110-40692.](http://images.novusbio.com/fullsize/USP33-Antibody-Western-Blot-NB110-40693-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: USP33 Antibody [NB110-40693] - Detection of Human USP33 on HeLa whole cell lysate using NB110-40693. USP33 was also immunoprecipitated by rabbit anti-USP33 antibody NB110-40692.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: USP33 Antibody [NB110-40693] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP33 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/USP33-Antibody-Immunohistochemistry-NB110-40693-img0005.jpg)
![Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/USP33-Antibody-Immunohistochemistry-Paraffin-NB110-40693-img0001.jpg)
![Immunohistochemistry-Paraffin: USP33 Antibody [NB110-40693] - FFPE section of mouse renal cell carcinoma. Affinity purified rabbit anti-USP33 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/USP33-Antibody-Immunohistochemistry-Paraffin-NB110-40693-img0002.jpg)
![Western Blot: USP14 Antibody [NBP2-20825] - Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20825-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: USP14 Antibody [NBP2-20825] - Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: USP14 Antibody [NBP2-20825] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/USP14-Antibody-Immunohistochemistry-Paraffin-NBP2-20825-img0004.jpg)
![Western Blot: USP14 Antibody [NBP2-20825] - Sample (30 ug of whole cell lysate) A: A431 B: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20825-img0001.jpg)
![Western Blot: USP14 Antibody [NBP2-20825] - USP14 antibody detects USP14 protein by Western blot analysis. A. 50 ug mouse brain lysate/extract7. 5 % SDS-PAGEUSP14 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20825-img0002.jpg)
![Western Blot: USP14 Antibody [NBP2-20825] - USP14 antibody detects USP14 protein by Western blot analysis. A. 50 ug rat brain lysate/extract7. 5 % SDS-PAGEUSP14 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20825-img0003.jpg)
![Western Blot: USP14 Antibody [NBP2-20825] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20825-img0005.jpg)
![Western Blot: USP14 Antibody [NBP2-20826] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20826-img0001.jpg "Western Blot: USP14 Antibody [NBP2-20826] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: USP14 Antibody [NBP2-20826] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/USP14-Antibody-Western-Blot-NBP2-20826-img0009.jpg)
![Immunohistochemistry-Paraffin: USP14 Antibody [NBP2-20826] - Human normal liver, using USP14 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/USP14-Antibody-Immunohistochemistry-Paraffin-NBP2-20826-img0003.jpg)
![USP14 antibody detects USP14 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP14 protein stained by USP14 antibody (NBP2-20826) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20826_rabbit-polyclonal-usp14-antibody-immunocytochemistry-immunofluorescence-2611202518321910.jpg)
![USP14 antibody detects USP14 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: USP14 protein stained by USP14 antibody (NBP2-20826) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-20826_rabbit-polyclonal-usp14-antibody-immunohistochemistry-paraffin-261120251833107.jpg)
![Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Western-Blot-H00023032-M01-img0011.jpg "Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Immunocytochemistry-Immunofluorescence-H00023032-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Immunohistochemistry-Paraffin-H00023032-M01-img0008.jpg)
![Western Blot: USP33 Antibody (5B5) [H00023032-M01] - USP33 expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Western-Blot-H00023032-M01-img0001.jpg)
![Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Immunoprecipitation-H00023032-M01-img0009.jpg)
![Sandwich ELISA: USP33 Antibody (5B5) [H00023032-M01] - Detection limit for recombinant GST tagged USP33 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/USP33-Antibody-5B5-Sandwich-ELISA-H00023032-M01-img0010.jpg)